DNA transfection

DNA transfection
A technique originally developed to allow viral infection of animal cells by uptake of purified viral DNA rather than by intact virus particles. Term, a hybrid between transformation and infection, is now generally used to describe applications of same methodology to introduction of other kinds of genes or gene fragments into cells as DNA, such as activated oncogenes from tumours into tissue culture cells.

Dictionary of molecular biology. 2004.

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  • Transfection — is the process of introducing nucleic acids into cells by non viral methods [http://www.promega.com/guides/transfxn guide/transfxn.pdf] . The term transformation is preferred to describe non viral DNA transfer in bacteria and non animal… …   Wikipedia

  • DNA vaccination — The making of a DNA vaccine. DNA vaccination is a technique for protecting an organism against disease by injecting it with genetically engineered DNA to produce an immunological response. Nucleic acid vaccines are still experimental, and have… …   Wikipedia

  • transfection — The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cell s chromosomal DNA. Usually accomplished using DNA precipitated with calcium ions though a variety of other methods can be used (eg.… …   Dictionary of molecular biology

  • transfection — A method of gene transfer utilizing infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell. [trans + infection] * * * trans·fec·tion …   Medical dictionary

  • transfection — noun Etymology: trans + infection Date: 1964 infection of a cell with isolated viral nucleic acid followed by production of the complete virus in the cell; also the incorporation of exogenous DNA into a cell • transfect transitive verb …   New Collegiate Dictionary

  • transfection — noun tɹænzˈfɛkʃən The introduction of foreign DNA into a eukaryotic cell. See Also: transfectant …   Wiktionary

  • transfection — n. the direct transfer of DNA molecules into a cell …   The new mediacal dictionary

  • transfection — The infection of a cell with isolated viral DNA (or RNA), resulting in the production of intact viral particles …   Glossary of Biotechnology

  • Optical transfection — is the process of introducing nucleic acids into cells using light. Typically, a laser is focussed to a diffraction limited spot ( 1 µm diameter) using a high numerical aperture microscope objective. The plasma membrane of a cell is then… …   Wikipedia

  • Magnet Assisted Transfection — Die Magnet Assisted Transfection (engl. Magnet unterstützte Transfektion ) ist eine Transfektionsmethode, die unter Ausnutzung eines magnetischen Feldes DNA in die Zielzellen einschleust. Dabei werden eisenhaltige, magnetische Nanopartikel mit… …   Deutsch Wikipedia

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